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Additionally, the vesicles within infected gro29 cells appeared similar to autophagosomes insofar as they contained a fantastic offer of cytoplasmic substance (Fig. 3B, arrows). We next investigated the status of autophagy in L and gro29 cells. Autophagosome formation calls for microtubule-connected protein 1 mild chain three (LC3), which is commonly utilized as a marker of autophagy [forty six].