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The cells have been developed to 80% confluence on the working day of transfection. The pEGFP-N1-Vpr encoding Vpr-GFP was transfected into concentrate on cells using jetPEI cationic polymer transfection reagent (Polyplus-transfection Inc., Illkirch, France). Human CD4+ T lymphoblast mobile line SupT1 was received from ATCC and grown in RPMI1640 medium supplemented with 10% FBS, 4 mM glutamine, one
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